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Effects of Hypoxia Exposure on Neurogenesis and the Hypothalamic-Pituitary-Interrenal (HPI) Axis in Larval Zebrafish (Danio rerio)

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Title: Effects of Hypoxia Exposure on Neurogenesis and the Hypothalamic-Pituitary-Interrenal (HPI) Axis in Larval Zebrafish (Danio rerio)
Author: Mikloska, Kristina
Department: Department of Integrative Biology
Program: Integrative Biology
Advisor: Bernier, Nicholas
Abstract: While the incidence of hypoxic waters is increasing globally, their impact on the development of the brain and on the activity of the hypothalamic-pituitary-interrenal stress axis in larval fish is not known. Therefore, the primary objective of this thesis was to determine how hypoxia exposure affects neurogenesis and the endocrine stress response in larval zebrafish (Danio rerio). Relative to normoxic controls, exposing 5 days post-fertilization (dpf) zebrafish larvae to 10% dissolved oxygen (DO), but not 20% DO, resulted in a 50% reduction in neural proliferation across the telencephalon, a reduction in neuronal precursor gene expression and a 2-fold increase in whole body cortisol. In contrast, exposing 5 dpf larvae to either 10 or 20% dpf reduced the cortisol response to a 10% DO exposure at 10 dpf. Overall, these results suggest that hypoxia is a significant stressor in larval zebrafish that may have persistent effects across developmental stages.
URI: http://hdl.handle.net/10214/8052
Date: 2014-05
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada