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Inhibition of Membrane-bound Lytic Transglycosylase A by Inhibitors of Vertebrate Lysozyme P1 and P2

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dc.contributor.advisor Clarke, Anthony
dc.contributor.author Selander, Iris
dc.date.accessioned 2014-04-04T18:46:31Z
dc.date.available 2014-04-04T18:46:31Z
dc.date.copyright 2014-04
dc.date.created 2014-03-24
dc.date.issued 2014-04-04
dc.identifier.uri http://hdl.handle.net/10214/7880
dc.description.abstract The proteinaceous inhibitors of vertebrate lysozyme (Ivy) p1 and p2 from Pseudomonas aeruginosa are two putative inhibitors of lytic transglycosylase (LT). The LTs are bacterial enzymes essential for the cleavage of glycosidic linkages in the peptidoglycan (PG) sacculus surrounding the cell. Although initially identified as inhibitors of lysozymes, Ivyp1 and Ivyp2 have previously been shown to inhibit a soluble derivative of membrane-bound LT B from P. aeruginosa. The current study investigated the inhibition of a soluble derivative of membrane-bound LT A (sMltA) from the same bacterium. Inhibition of sMltA by both Iyvp1 and Ivyp2 was confirmed in vitro using a turbidometric assay and the production and purification of all three proteins was improved upon. Thus, useful protocols were established for the continuation of the inhibition studies and more evidence was collected to support our hypothesis that Ivyp1 and Ivyp2, as well as homologs in other Gram-negative bacteria, are LT inhibitors. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject Peptidoglycan metabolism en_US
dc.subject Pseudomonas aeruginosa en_US
dc.subject Lytic transglycosylase en_US
dc.subject Inhibitor of vertebrate lysozyme en_US
dc.title Inhibition of Membrane-bound Lytic Transglycosylase A by Inhibitors of Vertebrate Lysozyme P1 and P2 en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada