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Inhibition of Membrane-bound Lytic Transglycosylase A by Inhibitors of Vertebrate Lysozyme P1 and P2

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Title: Inhibition of Membrane-bound Lytic Transglycosylase A by Inhibitors of Vertebrate Lysozyme P1 and P2
Author: Selander, Iris
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Clarke, Anthony
Abstract: The proteinaceous inhibitors of vertebrate lysozyme (Ivy) p1 and p2 from Pseudomonas aeruginosa are two putative inhibitors of lytic transglycosylase (LT). The LTs are bacterial enzymes essential for the cleavage of glycosidic linkages in the peptidoglycan (PG) sacculus surrounding the cell. Although initially identified as inhibitors of lysozymes, Ivyp1 and Ivyp2 have previously been shown to inhibit a soluble derivative of membrane-bound LT B from P. aeruginosa. The current study investigated the inhibition of a soluble derivative of membrane-bound LT A (sMltA) from the same bacterium. Inhibition of sMltA by both Iyvp1 and Ivyp2 was confirmed in vitro using a turbidometric assay and the production and purification of all three proteins was improved upon. Thus, useful protocols were established for the continuation of the inhibition studies and more evidence was collected to support our hypothesis that Ivyp1 and Ivyp2, as well as homologs in other Gram-negative bacteria, are LT inhibitors.
URI: http://hdl.handle.net/10214/7880
Date: 2014-04
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada