Main content

A platform upgrade will be performed on the Atrium Institutional Repository from Monday, July 13 to Wenesday, July 15, 2020 (inclusive). During this time, users will not be able to submit new items to the Atrium. Users will still be able to browse, view, and download items that are already available in the Atrium. We apologize for any inconvenience this may cause.

Immobilization of bacteriophages for the control and detection of food-borne pathogens

Show simple item record

dc.contributor.advisor Griffiths, Mansel
dc.contributor.author Sohar, Jennifer
dc.date.accessioned 2014-01-15T15:21:43Z
dc.date.available 2015-01-10T06:00:16Z
dc.date.copyright 2014-01
dc.date.created 2014-01-10
dc.date.issued 2014-01-15
dc.identifier.uri http://hdl.handle.net/10214/7823
dc.description.abstract Immobilized phages may provide diverse opportunities to control and detect food-borne bacterial pathogens. Piezoelectric printing was used to immobilize phages AG6, AG10, AG11 and AG20 on ColorLok paper for the development of bioactive packaging material. The individual phage-based papers resulted in log10 reductions in count of 0.28, 2.68, 2.11 and 3.89 cfu/ml for Salmonella Typhimurium, Salmonella Enteritidis, Escherichia coli O157:H7 and Listeria monocytogenes, respectively. The printed phage-based paper stored at room temperature and 80-85% RH was not stable after one month. Phage amplification assays for rapid detection of E. coli O157:H7 were developed using rV5 phage electrostatically immobilized on APTS modified magnetic silica beads. Detection limits were 11-25 cfu/ml for a continuous phage amplification assay and 100-200 cfu/ml in a phage capture-amplification assay. Results were obtained within approximately 8 h. Immobilized phages have great potential to help ensure food safety. en_US
dc.description.sponsorship Sentinel Bioactive Paper Network en_US
dc.language.iso en en_US
dc.subject Immobilized bacteriophages en_US
dc.subject Printing of phages en_US
dc.subject Coating of phages en_US
dc.subject Electrostatic phage immobilization en_US
dc.subject phage amplification assay en_US
dc.subject phage-based packaging material en_US
dc.subject phage-coated beads en_US
dc.title Immobilization of bacteriophages for the control and detection of food-borne pathogens en_US
dc.type Thesis en_US
dc.degree.programme Food Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Food Science en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Sohar_Jennifer_201401_Msc.pdf 1.978Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record