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Immobilization of bacteriophages for the control and detection of food-borne pathogens

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dc.contributor.advisor Griffiths, Mansel Sohar, Jennifer 2014-01-15T15:21:43Z 2015-01-10T06:00:16Z 2014-01 2014-01-10 2014-01-15
dc.description.abstract Immobilized phages may provide diverse opportunities to control and detect food-borne bacterial pathogens. Piezoelectric printing was used to immobilize phages AG6, AG10, AG11 and AG20 on ColorLok paper for the development of bioactive packaging material. The individual phage-based papers resulted in log10 reductions in count of 0.28, 2.68, 2.11 and 3.89 cfu/ml for Salmonella Typhimurium, Salmonella Enteritidis, Escherichia coli O157:H7 and Listeria monocytogenes, respectively. The printed phage-based paper stored at room temperature and 80-85% RH was not stable after one month. Phage amplification assays for rapid detection of E. coli O157:H7 were developed using rV5 phage electrostatically immobilized on APTS modified magnetic silica beads. Detection limits were 11-25 cfu/ml for a continuous phage amplification assay and 100-200 cfu/ml in a phage capture-amplification assay. Results were obtained within approximately 8 h. Immobilized phages have great potential to help ensure food safety. en_US
dc.description.sponsorship Sentinel Bioactive Paper Network en_US
dc.language.iso en en_US
dc.subject Immobilized bacteriophages en_US
dc.subject Printing of phages en_US
dc.subject Coating of phages en_US
dc.subject Electrostatic phage immobilization en_US
dc.subject phage amplification assay en_US
dc.subject phage-based packaging material en_US
dc.subject phage-coated beads en_US
dc.title Immobilization of bacteriophages for the control and detection of food-borne pathogens en_US
dc.type Thesis en_US Food Science en_US Master of Science en_US Department of Food Science en_US
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