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Immobilization of bacteriophages for the control and detection of food-borne pathogens

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Title: Immobilization of bacteriophages for the control and detection of food-borne pathogens
Author: Sohar, Jennifer
Department: Department of Food Science
Program: Food Science
Advisor: Griffiths, Mansel
Abstract: Immobilized phages may provide diverse opportunities to control and detect food-borne bacterial pathogens. Piezoelectric printing was used to immobilize phages AG6, AG10, AG11 and AG20 on ColorLok paper for the development of bioactive packaging material. The individual phage-based papers resulted in log10 reductions in count of 0.28, 2.68, 2.11 and 3.89 cfu/ml for Salmonella Typhimurium, Salmonella Enteritidis, Escherichia coli O157:H7 and Listeria monocytogenes, respectively. The printed phage-based paper stored at room temperature and 80-85% RH was not stable after one month. Phage amplification assays for rapid detection of E. coli O157:H7 were developed using rV5 phage electrostatically immobilized on APTS modified magnetic silica beads. Detection limits were 11-25 cfu/ml for a continuous phage amplification assay and 100-200 cfu/ml in a phage capture-amplification assay. Results were obtained within approximately 8 h. Immobilized phages have great potential to help ensure food safety.
URI: http://hdl.handle.net/10214/7823
Date: 2014-01


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