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Influence of O Polysaccharides on Outer Membrane Vesicle Formation and Biofilm Development in Pseudomonas aeruginosa PAO1

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Title: Influence of O Polysaccharides on Outer Membrane Vesicle Formation and Biofilm Development in Pseudomonas aeruginosa PAO1
Author: Murphy, Kathleen
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Khursigara, Cezar
Abstract: Certain environmental conditions can alter the expression of the lipopolysaccharide (LPS) O polysaccharide on the surface of Pseudomonas aeruginosa cells. Changes in O polysaccharide expression influence cell surface characteristics. This study investigated the effect of directly altering O polysaccharide expression on outer membrane vesicle (OMV) production and composition, as well as biofilm formation. P. aeruginosa PAO1 produces two structurally distinct forms of O polysaccharide, the common polysaccharide antigen (CPA) and the O-specific antigen (OSA). Chromosomal knockout mutants of PAO1 that are defective in producing either OSA (∆wbpM) or CPA (∆rmd), or both (∆wbpL) were compared to wildtype cells. It was demonstrated that changes in O polysaccharide expression do not significantly influence OMV biogenesis, but do affect the size and protein sorting of OMVs. In addition, O polysaccharide expression was determined to influence biofilm development, and results from this study highlight the importance of CPA in this process.
URI: http://hdl.handle.net/10214/7803
Date: 2014-01
Rights: Attribution-NoDerivs 2.5 Canada
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Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada