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Cytokine Gene Expression in Holstein-Friesian and Jersey Calves Infected with Mycobacterium avium subsp. paratuberculosis

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dc.contributor.advisor Karrow, Niel
dc.contributor.author Mallikarjunappa, Sanjay
dc.date.accessioned 2014-01-06T21:43:38Z
dc.date.available 2014-01-06T21:43:38Z
dc.date.copyright 2013-12
dc.date.created 2013-12-20
dc.date.issued 2014-01-06
dc.identifier.uri http://hdl.handle.net/10214/7761
dc.description.abstract Breed susceptibility to bovine Johne’s disease has been implied suggesting that Jersey cattle are more susceptible to disease as compared to Holstein-Friesian. A sixty-day experimental in vivo infection study was carried out by surgically infecting Holstein-Friesian and Jersey calves with the etiological agent of Johne’s disease, Mycobacterium avium subsp. paratuberculosis (Map). Breed-specific comparison of expression of key cytokine transcripts in the ileocecal lymph nodes of Map-infected and uninfected calves by real-time PCR did not indicate any significant differences. The expression of IFN-γ transcript was found to be significantly different for the treatment groups in both breeds with a higher expression level in infected than in control animals but there was no indication this differed between breeds. These data indicate that there are no breed-specific differences in the expression of key cytokine transcripts at sixty days post Map-infection and warrants long-term infection studies to analyze breed-specific immune response and in defining breed susceptibility. en_US
dc.description.sponsorship Natural Sciences and Engineering Research Council of Canada (NSERC), DairyGen, Dairy Farmers of Ontario en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject Johne's disease en_US
dc.subject Cytokine gene expression en_US
dc.subject Breed susceptibility en_US
dc.title Cytokine Gene Expression in Holstein-Friesian and Jersey Calves Infected with Mycobacterium avium subsp. paratuberculosis en_US
dc.type Thesis en_US
dc.degree.programme Animal and Poultry Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Animal and Poultry Science en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada