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Resistance to Verticillium in Tomatoes: the Root-Stem Controversy

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dc.contributor.advisor Robb, Jane
dc.contributor.author Mackey, Melora
dc.date.accessioned 2014-01-04T18:30:18Z
dc.date.available 2014-01-04T18:30:18Z
dc.date.copyright 2013-12
dc.date.created 2013-12-20
dc.date.issued 2014-01-04
dc.identifier.uri http://hdl.handle.net/10214/7749
dc.description.abstract Verticillium is a soil-borne fungus that is one of the world's foremost plant pathogens. Commercial plant grafting suggests that resistance occurs in the root; this conflicts with decades of research indicating that resistance occurs in the stem. The goal of this thesis work was to use an alternative approach to determine the location of resistance by expressing the Ve1 gene using organ-specific promoters. Promoter sequences for the stem-specific gene, Ribulose 1,5-bisphosphate carboxylase oxygenase small chain 2A (Rbsc2A), and root-specific gene, Tobacco Mosaic Virus Induced (TMVi) were taken from the Sol Genomics Network (SGN) database, cloned into constructs with the Ve1 gene and susceptible tomato germplasm was transformed using Agrobacterium tumefaciens. Preliminary results suggest that resistance may not be localized and expression of the Ve1 gene in either the root or the stem is sufficient to develop whole plant resistance to the Verticillium pathogen. en_US
dc.language.iso en en_US
dc.subject Tomato en_US
dc.subject promoter en_US
dc.subject organ-specific promoter en_US
dc.subject Rubisco en_US
dc.subject Tobacco Mosaic Virus Induced en_US
dc.subject Verticillium en_US
dc.subject Ve1 en_US
dc.subject Resistance en_US
dc.subject Plant-Pathogen Interaction en_US
dc.title Resistance to Verticillium in Tomatoes: the Root-Stem Controversy en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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