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Fabrication of Tobacco Mosaic Virus Imprinted Polymer on the Surface of Paramagnetic Nanobeads via Surface-Initiated Atom Transfer Radical Polymerization

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dc.contributor.advisor Warriner, Keith
dc.contributor.author Sun, Ran
dc.date.accessioned 2013-12-10T16:37:46Z
dc.date.available 2013-12-10T16:37:46Z
dc.date.copyright 2013-12
dc.date.created 2013-11-27
dc.date.issued 2013-12-10
dc.identifier.uri http://hdl.handle.net/10214/7695
dc.description.abstract Tobacco mosaic virus (TMV) imprinted polymer was grafted onto the surface of paramagnetic nanobeads via atom transfer radical polymerization (ARTP). The affinity of the imprinted paramagnetic nanobeads towards TMV was dependent on the polymer nucleation sites and cross-linker concentration included in the polymerization mix. Greater TMV binding to imprinted nanobeads (0.235 mg/ml) over non-imprinted controls (0.100 mg/ml) was observed at pH 7.0 and short (5 min) binding times. Using the TMV imprinted nanobeads, it was possible to extract and concentrate TMV particles from solutions containing as little as 100 μg/ml of the virus in buffered solutions. The imprinted beads were highly discriminative and could selectivity bind TMV over the structurally similar virus, Pepper Mild Mottle Virus. The results provide proof-of-principle of an approach for fabricating microbial imprinted paramagnetic nanobeads that could find utility as an alternative to immuno-assays in pathogen screening. en_US
dc.description.sponsorship the National Center for Food Protection and Defense en_US
dc.language.iso en en_US
dc.rights Attribution 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by/2.5/ca/ *
dc.subject Tobacco mosaic virus en_US
dc.subject paramagnetic nanobeads en_US
dc.subject Molecular imprinted polymer en_US
dc.subject Atom transfer radical polymerization en_US
dc.title Fabrication of Tobacco Mosaic Virus Imprinted Polymer on the Surface of Paramagnetic Nanobeads via Surface-Initiated Atom Transfer Radical Polymerization en_US
dc.type Thesis en_US
dc.degree.programme Food Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Food Science en_US


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Attribution 2.5 Canada Except where otherwise noted, this item's license is described as Attribution 2.5 Canada