Main content

Control of the Endocrine Stress Response in Goldfish (Carassius auratus)

Show simple item record

dc.contributor.advisor Bernier, Nicholas J.
dc.contributor.author Lim, Jan Edward
dc.date.accessioned 2013-09-19T14:29:29Z
dc.date.available 2013-09-19T14:29:29Z
dc.date.copyright 2013-09
dc.date.created 2013-09-13
dc.date.issued 2013-09-19
dc.identifier.uri http://hdl.handle.net/10214/7553
dc.description.abstract The purpose of this study was to obtain a deeper understanding the serotonergic control of the hypothalamic-pituitary-interrenal (HPI) stress axis and characterize a neuronal and behavioural response to a known stressor, using goldfish (Carassius auratus) as a model. The first part of this study investigated the serotonergic control of the HPI stress axis. In vivo injections of the serotonin (5-HT1A/7) receptor agonist 8-OH-DPAT resulted in significant and sustained increases in plasma cortisol without any changes in the mRNA abundance of brain corticotropin-releasing factor (CRF) and pituitary pro-opiomelanocortin or plasma adrenocorticotropic hormone levels. In vitro superfusion experiments of head kidney tissue showed that 5-HT can directly stimulate cortisol synthesis from the interrenals via several 5-HT receptor subtypes. Finally, double-labeling with anti-tyrosine hydroxylase and anti-5-HT revealed that the chromaffin cells of the head kidney contain 5-HT. The second part of the study investigated the goldfish response to a known stressor, the alarm pheromone. Alarm pheromone exposure resulted in an inhibition of movement and exploration and in suppression of neuronal activity in the pars parvocellularis of the nucleus preopticus, a brain region with extensive CRF immunolabelling. Overall, the results from this thesis suggest that in goldfish 5-HT may be involved in the paracrine regulation of cortisol secretion and that the CRF neurons of the pars parvocellularis play a role in mediating the stress response to alarm pheromones. en_US
dc.language.iso en en_US
dc.subject Serotonin en_US
dc.subject Cortisol en_US
dc.subject HPI axis en_US
dc.subject Stress en_US
dc.subject Interrenal cells en_US
dc.subject Chromaffin cells en_US
dc.subject Alarm pheromones en_US
dc.subject CRF en_US
dc.title Control of the Endocrine Stress Response in Goldfish (Carassius auratus) en_US
dc.type Thesis en_US
dc.degree.programme Integrative Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Integrative Biology en_US


Files in this item

Files Size Format View
Lim_Jan_201309_Msc.pdf 1.893Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record