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Factors Affecting the Efficacy of Commercial Post-harvest Washing of Fresh-cut Leafy Greens and the Dynamics of Microbial Populations within a Commercial Wash Process

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dc.contributor.advisor Warriner, Keith
dc.contributor.author Zahedi, Hamed
dc.date.accessioned 2013-09-13T15:35:21Z
dc.date.available 2013-09-13T15:35:21Z
dc.date.copyright 2013-09
dc.date.created 2013-09-09
dc.date.issued 2013-09-13
dc.identifier.uri http://hdl.handle.net/10214/7533
dc.description.abstract Produce washing remains a key intervention despite only achieving a 1-2 log reduction and potential for cross-contamination. Our objectives were to establish the wash water parameters that impact on the efficacy of washing leafy greens within commercial settings. Sampling visits were performed at two commercial fresh-cut processors with the microbial loading of raw material, pre-wash and post-wash product being determined. At the same time water quality parameters (bacterial loading, turbidity, conductivity, and temperature) were measured. Microbial community populations were evaluated using Eco-plates TM and 16S rDNA. From the multiple parameters tested, the LCR could be correlated to turbidity and ORP. Interestingly, the LCR was independent of microbial loading of the water yet increased levels correlated with cross-contamination events as observed using microbial population profiling. Washing of leafy greens is limited and alternative treatments should focus on how to minimize cross-contamination as opposed to focusing on log count reductions. en_US
dc.description.sponsorship Center for Produce Safety (CPS) en_US
dc.language.iso en en_US
dc.rights Attribution-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nd/2.5/ca/ *
dc.subject Washing efficacy en_US
dc.subject Monitoring quality of wash water en_US
dc.subject Microbial community en_US
dc.subject APC en_US
dc.subject Coliform en_US
dc.subject Lettuce en_US
dc.subject Spinach en_US
dc.subject Leafy green en_US
dc.subject Frsh-cut produce en_US
dc.subject Cross-contaminatin en_US
dc.subject Pre-washing en_US
dc.subject Biocidal tank en_US
dc.subject AC impedance en_US
dc.subject Turbidity en_US
dc.subject ORP en_US
dc.subject pH en_US
dc.subject 16 S rDNA en_US
dc.subject 16 S rRNA en_US
dc.subject Ecoplate en_US
dc.subject Metabolic fingerprint en_US
dc.subject Bacterial DNA fingerprint en_US
dc.subject Food Safety en_US
dc.subject CCP en_US
dc.subject Sanitizer en_US
dc.subject Chlorine en_US
dc.subject PAA en_US
dc.subject Peracetic Acid en_US
dc.subject LCR en_US
dc.subject Microbial deposition en_US
dc.title Factors Affecting the Efficacy of Commercial Post-harvest Washing of Fresh-cut Leafy Greens and the Dynamics of Microbial Populations within a Commercial Wash Process en_US
dc.type Thesis en_US
dc.degree.programme Food Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Food Science en_US
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Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada