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Infection of monocyte-derived macrophages with a reporter Map strain: Validation of the susceptibilty SNP (-298A/G) in the bovine MIF gene

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dc.contributor.advisor Niel, Karrow
dc.contributor.author Mead, Philip James
dc.date.accessioned 2013-09-10T20:31:43Z
dc.date.available 2013-09-10T20:31:43Z
dc.date.copyright 2013-09
dc.date.created 2013-08-19
dc.date.issued 2013-09-10
dc.identifier.uri http://hdl.handle.net/10214/7503
dc.description.abstract Johne’s disease (JD) is a chronic, infectious enteritis of ruminant animals caused by Mycobacterium avium subsp. paratuberculosis (Map). Monitoring host-pathogen interaction over time is critical to understanding the complex pathogenesis of JD. A major constraint in studying the host-pathogen interaction in JD infection is the inherent difficulty of detecting cells infected with Map. The purpose of the present study was to develop and use a fluorescent Map isolate to assess the association between SNP (-298 A>G) in the MIF gene with innate susceptibility to Map infection. In order to rapidly detect bacterial load within host cells, we cloned the gene encoding mCherry into a clinical isolate of Map. The recombinant Map clinical isolate expressing mCherry was used to quantify bacterial numbers, detect infected macrophages in vitro using fluorescent microscopy, determine effector killing of bacteria by macrophages and was tested in an in vivo calf infection study to assess its stability. en_US
dc.description.sponsorship Dairy Farmers of Ontario, National Sciences and Engineering Research Council of Canada, Ontario Ministry of Agriculture, Food and Rural Affairs, Canwest DHI, CDN, Canada Foundation for Innovation en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject Johnes Disease en_US
dc.subject Mycobacterium avium subsp paratuberculosis en_US
dc.title Infection of monocyte-derived macrophages with a reporter Map strain: Validation of the susceptibilty SNP (-298A/G) in the bovine MIF gene en_US
dc.type Thesis en_US
dc.degree.programme Animal and Poultry Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Animal and Poultry Science en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada