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Quantifying Bacterial Crowd Surfing in Pseudomonas aeruginosa

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dc.contributor.advisor Dutcher, John Robert
dc.contributor.author Moscaritolo, Robert Frank
dc.date.accessioned 2013-09-09T16:38:42Z
dc.date.available 2013-09-09T16:38:42Z
dc.date.copyright 2013-08
dc.date.created 2013-08-29
dc.date.issued 2013-09-09
dc.identifier.uri http://hdl.handle.net/10214/7489
dc.description.abstract Type IV pili (T4P) are adhesive protein filaments that can be extended and retracted by certain classes of Gram-negative bacteria. The motion of bacteria on surfaces by T4P is referred to as twitching motility, and it has been shown that this leads to complex, collective motion of large numbers of cells. We have observed that twitching cells of P. aeruginosa PAO1 could transport inert micrometre-sized objects (“crowd surfing”). Crowd surfing also extends to non-motile bacterial cells, and can lead to the transport of pathogenic bacterial cells, with direct implications for the spread of bacterial infections. We have designed and constructed a custom-built environmental chamber to quantify this phenomenon under precisely controlled environmental conditions. We found that the twitching bacterial cells drive the motion of the microspheres with a well-defined average speed, and that the directionality of their motion remains highly correlated for the largest time scales observed in the experiments. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.title Quantifying Bacterial Crowd Surfing in Pseudomonas aeruginosa en_US
dc.type Thesis en_US
dc.degree.programme Biophysics en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Physics en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada