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EPA and DHA Modulate Macrophage-Derived Inflammation and Subsequent Skeletal Muscle Inflammation

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dc.contributor.advisor Robinson, Lindsay
dc.contributor.author Sepa-Kishi, Diane
dc.date.accessioned 2013-09-07T20:28:51Z
dc.date.available 2013-09-07T20:28:51Z
dc.date.copyright 2013-08
dc.date.created 2013-08-29
dc.date.issued 2013-09-07
dc.identifier.uri http://hdl.handle.net/10214/7484
dc.description.abstract Macrophage-derived inflammation contributes to chronic inflammation in adipose tissue in obesity and is also linked to the development of skeletal muscle (SM) insulin resistance. The long-chain n-3 PUFA have been shown to modulate cytokine secretion from macrophages, though subsequent effects on SM inflammation and function are unknown. A model of macrophage conditioned media (MCM) was used to examine effects of n-3 PUFA on macrophage inflammation and consequent effects on SM cells. Treatment of RAW 264.7 macrophages with long-chain n-3 PUFA decreased LPS-induced MCP-1 and IL-6 gene expression and MCP-1 secreted protein. In turn, MCM from n-3 PUFA-treated macrophages decreased TNF-α and IL-6 gene expression in LPS-stimulated L6 SM cells, but did not affect insulin-stimulated pAkt content. Long-chain n-3 PUFA did not affect gene expression of inflammatory signaling intermediates NF-κB and TLR4. Overall this thesis suggests that long-chain n-3 PUFA are important nutritional strategies for reducing macrophage-derived inflammation, with ensuing benefits in SM inflammation. en_US
dc.description.sponsorship NSERC-CGS, Ontario Graduate Scholarship en_US
dc.language.iso en en_US
dc.subject macrophage-derived inflammation en_US
dc.subject adipose tissue en_US
dc.subject skeletal muscle en_US
dc.subject n-3 polyunsaturated fatty acids en_US
dc.subject macrophage conditioned media en_US
dc.subject LPS-induced inflammation en_US
dc.title EPA and DHA Modulate Macrophage-Derived Inflammation and Subsequent Skeletal Muscle Inflammation en_US
dc.type Thesis en_US
dc.degree.programme Human Health and Nutritional Sciences en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Human Health and Nutritional Sciences en_US
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