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Molecular Mapping and Characterization of Phenylpropanoid Pathway Genes in Common Bean (Phaseolus vulgaris L.)

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Title: Molecular Mapping and Characterization of Phenylpropanoid Pathway Genes in Common Bean (Phaseolus vulgaris L.)
Author: Yadegari, Zeinab
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: Pauls, K Peter
Abstract: Common bean is a nutritionally and economically important food crop and a major source of dietary protein in many developing countries throughout the world. Seed coat colour and size in this crop are the main factors determining its marketability in different parts of the world. Flavonoid compounds that are responsible for seed coat colour in beans have been shown to have anti-oxidant, anti-proliferative, anti-tumor, anti-inflammatory, and pro-apoptotic activities. They also may enhance the resistance of beans to pest and disease. A better understanding of the relationships between seed coat colour and flavonoid metabolism in the seed coat may help breeders to select for more nutritionally-beneficial bean varieties. The objective of this research was to test the hypothesis that the genes determining colour in beans are structural and regulatory genes of the phenylpropanoid pathway. The map positions of phenylpropanoid genes were determined in two recombinant inbred populations. Segregation patterns of 18 phenylpropanoid pathway genes in the BAT93 × Jalo EEP 558 RIL population and five phenylpropanoid pathway genes in OAC Rex × SVM Taylor were used to place them on the linkage maps for these populations. Five out of 18 genes were mapped within 2-17 cM of colour gene loci in the BAT93 × Jalo EEP 558 RIL population. The sequences of central genes of the phenylpropanoid pathway were determined by sequencing 6 BAC clones selected with probes for two PAL genes, two CHS genes, DFR, and Myb. The functional annotations of the BAC clones were determined and the similarities between bean phenylpropanoid genes and their corresponding orthologs in other plant species were investigated. A recently developed approach of whole genome sequence comparison was utilized to compare the microsynteny of the sequenced BAC clones with regions of the soybean genome. The physical locations of BAC clones were verified on the bean genome and their counterpart locations on the soybean genome were confirmed. The results agreed with previous studies that indicated that bean genome segments have two homologous segments in soybean and confirmed the high degree of microsynteny that is shared between bean and soybean.
URI: http://hdl.handle.net/10214/7471
Date: 2013-06
Rights: Attribution-NonCommercial-ShareAlike 2.5 Canada


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Attribution-NonCommercial-ShareAlike 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 2.5 Canada