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Detection and quantification of Cryptosporidium oocysts in environmental samples

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dc.contributor.advisor Lee, Hung
dc.contributor.advisor Trevors, Jack T
dc.contributor.advisor Weir, Susan
dc.contributor.author Duggal, Megha
dc.date.accessioned 2013-08-30T19:31:19Z
dc.date.available 2013-08-30T19:31:19Z
dc.date.copyright 2013-08
dc.date.created 2013-08-27
dc.date.issued 2013-08-30
dc.identifier.uri http://hdl.handle.net/10214/7443
dc.description.abstract A PCR Differentiation Method, a hybrid of the US EPA and MOE methods, for quantifying human infectious C. parvum/C. hominis as a group and non-human infectious C. andersoni/C. muris was developed. Primers and probe sets targeting the hsp70 gene were designed for C. andersoni/C. muris; those for C. parvum/C. hominis were obtained from the MOE method. Results showed that C, andersoni/C. muris primers were specific for C. andersoni/C. muris oocysts, while those for C. parvum/hominis primers detected C. parvum/hominis and C. meleagridis. All primers were then used to quantify oocysts from urban and agricultural environmental water samples in Kitchener/Waterloo. Human infectious Giardia lamblia was also incorporated into this study. C. parvum/C. hominis and Giardia lamblia were detected at urban and agricultural areas, whereas C. andersoni/C. muris was only detected at agricultural sites. The PCR Differentiation Method is a reliable method for quantifying Cryptosporidium and Giardia lamblia in environmental water samples. en_US
dc.description.sponsorship Best in Science Program of the Ontario Ministry of the Environment, Natural Sciences and Engineering Council (NSERC) of Canada Discovery Grants en_US
dc.language.iso en en_US
dc.rights Attribution-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nd/2.5/ca/ *
dc.subject Cryptosporidium en_US
dc.subject detection en_US
dc.subject PCR en_US
dc.subject real time en_US
dc.subject environment en_US
dc.title Detection and quantification of Cryptosporidium oocysts in environmental samples en_US
dc.type Thesis en_US
dc.degree.programme Environmental Sciences en_US
dc.degree.name Master of Science en_US
dc.degree.department School of Environmental Sciences en_US
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Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada