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The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus)

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dc.contributor.advisor Parker, Linda
dc.contributor.author O'Brien, Lesley D
dc.date.accessioned 2013-08-07T13:52:37Z
dc.date.available 2013-08-07T13:52:37Z
dc.date.copyright 2013-07
dc.date.created 2013-07-17
dc.date.issued 2013-08-07
dc.identifier.uri http://hdl.handle.net/10214/7296
dc.description.abstract Considerable evidence supports anandamide (AEA) as an important mediator in the regulation of nausea and vomiting. The present study investigates the effect of inhibiting a protein reported to mediate AEA transport, FLAT (FAAH-1-like AEA transporter), on nausea and vomiting and the neural correlates of AEA regulated nausea in the visceral insular cortex (VIC). The systemic administration of the AEA transport inhibitor ARN272 was evaluated in LiCl-induced conditioned gaping in rats, and vomiting in shrews. The effect of intra-cranial administration of ARN272 into the VIC was also investigated using LiCl-induced conditioned gaping in rats. Systemic administration of ARN272 dose-dependently suppressed LiCl-induced conditioned gaping in rats, and was reversed by CB1 receptor antagonism with SR141716. Systemic administration of ARN272 also attenuated vomiting in shrews. Delivery of ARN272 into the VIC produced no effect on LiCl-induced conditioned gaping in rats. These results suggest that preventing the cellular reuptake of AEA through transport inhibition tonically activates CB1 receptors to regulate toxin-induced nausea, but that this is not AEA regulated within the VIC. en_US
dc.description.sponsorship This research was supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC-92057) to LAP. en_US
dc.language.iso en en_US
dc.subject Endocannabinoid en_US
dc.subject Anandamide en_US
dc.subject Nausea en_US
dc.subject Gaping en_US
dc.subject Vomiting en_US
dc.subject FAAH-1-like anandamide transporter en_US
dc.subject FLAT en_US
dc.subject CB1 en_US
dc.subject ARN272 en_US
dc.subject taste reactivity en_US
dc.subject Visceral Insular Cortex en_US
dc.title The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus) en_US
dc.type Thesis en_US
dc.degree.programme Psychology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Psychology en_US


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