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The influence of Toll-like receptors on murine invariant natural killer T cell activation

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dc.contributor.advisor Sharif, Shayan Villanueva, Alexander Ian 2013-06-21T18:53:10Z 2013-06-21T18:53:10Z 2013-06 2013-06-14 2013-06-21
dc.description.abstract Invariant natural killer T (iNKT) cells are a versatile subclass of T lymphocytes which recognize glycolipid antigens. iNKT cells are capable of rapidly producing a broad array of cytokines in response to stimulation; thus, they play an important role in the early regulation of a variety of immune responses. It was hypothesized that iNKT cells express functional Toll-like receptors (TLRs) and that stimulation of TLRs by their ligands modulates iNKT cells responses. In the first objective, it was revealed that upon stimulation with anti-CD3 monoclonal antibody and interferon (IFN)-α, expression of TLRs was enhanced in iNKT cells. Furthermore, stimulation of iNKT cells with TLR ligands led to a significant increase in the expression of several cytokines. In the second objective, the mechanisms behind the modulatory effects of the TLR9 ligand (CpG-ODN) on iNKT cells were determined. Altogether, these findings suggest a direct role for TLRs in iNKT cell activation. en_US
dc.description.sponsorship Ontario Graduate Scholarship en_US
dc.language.iso en en_US
dc.subject immunology en_US
dc.subject NKT cells en_US
dc.subject innate immunity en_US
dc.subject Toll-like receptors en_US
dc.subject real-time PCR en_US
dc.subject ELISA en_US
dc.subject Western blot en_US
dc.subject flow cytometry en_US
dc.subject macrophage en_US
dc.subject cell lines en_US
dc.subject hybridoma en_US
dc.subject VSV en_US
dc.subject MAPK en_US
dc.subject MAPK phosphatase en_US
dc.subject DUSP1 en_US
dc.subject CpG en_US
dc.subject T cells en_US
dc.subject natural killer cells en_US
dc.subject gene expression en_US
dc.title The influence of Toll-like receptors on murine invariant natural killer T cell activation en_US
dc.type Thesis en_US Pathobiology en_US Master of Science en_US Department of Pathobiology en_US
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