Main content

Understanding the Role of the Arp2/3 Complex and its Upstream Regulator in Actin Cytoskeleton Mediated Organization of the Endoplasmic Reticulum in Plant Cells

Show simple item record

dc.contributor.advisor Mathur, Jaideep
dc.contributor.author sareen, madhulika
dc.date.accessioned 2013-05-10T14:48:29Z
dc.date.available 2013-05-10T14:48:29Z
dc.date.copyright 2013-05
dc.date.created 2013-05-01
dc.date.issued 2013-05-10
dc.identifier.uri http://hdl.handle.net/10214/6664
dc.description.abstract The Actin Related Protein (ARP) 2/3 complex is a major regulator of the actin cytoskeleton that is implicated in cell morphogenesis in plants. However, a similar role is attributed to the endoplasmic reticulum (ER). My research explored the relationship between the two systems by using transgenic plants simultaneously expressing fluorescent proteins highlighting F-actin and ER organization in living cells. A comparison of F-actin organization in cells of wild type Arabidopsis thaliana and mutants with aberrant actin cytoskeleton suggests bundling in the distorted2 mutant but a relatively fine F-actin arrangement in klunker. These differences correlate with ER organization into cisternae, fenestrated sheets and tubules. A model relating ER-organization to the degree of actin bundling in a cell emerges and is supported by drug-induced interference in actin polymerization, altered ionic conditions and temperature. The study adds to the mechanistic understanding of cell morphogenesis in plants. en_US
dc.language.iso en en_US
dc.subject F-actin en_US
dc.subject Endoplasmic reticulum en_US
dc.subject ARP2/3 complex en_US
dc.title Understanding the Role of the Arp2/3 Complex and its Upstream Regulator in Actin Cytoskeleton Mediated Organization of the Endoplasmic Reticulum in Plant Cells en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US


Files in this item

Files Size Format View
sareen_madhulika_201305_Msc.pdf 2.082Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record