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The iFat-1 Transgene Permits Conditional Endogenous n-3 Polyunsaturated Fatty Acid Enrichment both in vitro and in vivo

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Title: The iFat-1 Transgene Permits Conditional Endogenous n-3 Polyunsaturated Fatty Acid Enrichment both in vitro and in vivo
Author: Clarke, Shannon
Department: Department of Human Health and Nutritional Sciences
Program: Human Health and Nutritional Sciences
Advisor: Ma, David
Abstract: Based on their highly bioactive properties in membrane phospholipids, there is growing recognition that dietary n-3 polyunsaturated fatty acids (PUFA) may be of significant benefit in the prevention and treatment of many lifestyle related pathologies, however direct evidence is lacking. The fat-1 transgenic mouse, a genetic model of n-3 PUFA enrichment, is a useful tool in nutritional research which has provided enhanced insight into the health effects of lifelong n-3 PUFA exposure. However, the influence of timing of n-3 PUFA exposure on health related outcomes remains unclear. This thesis describes the functional characterization of the novel Cre recombinase dependent inducible fat-1 (iFat-1) transgene. In the presence of Cre, the iFat-1 transgene was found to reduce phospholipid n-6/n-3 PUFA ratios both in vitro (100%) and in vivo (upwards of 70%), suggesting that the iFat-1 transgene has potential application to address temporal effects of n-3 PUFA in health and disease.
URI: http://hdl.handle.net/10214/5334
Date: 2013-01
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