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Understanding the Cellular Mechanisms Responsible for Blood Glucose Modulation By Oat Beta-glucan

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Title: Understanding the Cellular Mechanisms Responsible for Blood Glucose Modulation By Oat Beta-glucan
Author: Abbasi, Nazanin Nadia
Department: Department of Food Science
Program: Food Science
Advisor: Purslow, Peter
Abstract: The aim of this study was to understand the cellular mechanisms in enterocytes, which may decrease glucose uptake by viscous oat β-glucan. An in-vitro cell model examined the effect of diffusion limitation, fluid shear stimulation, and increased intestinal stretching. Mechanical stimulation of IEC-6 was assessed. A Flexcell Cell Streamer device applied different fluid flow stresses on cells. Flexcell FX-4000 was used for biaxial stretching of the cells. Following the confirmation of appropriate use of the cell model, the results indicated that high viscosity oat β-glucan might provide a physical barrier limiting diffusion of nutrients to the cells apical surfaces. Western blot analysis confirmed weak mechanical stimulation on the cells. Mechanical stimulation did not influence glucose uptake. Strain-induced cells showed lower activities in their glucose uptake. In conclusion, there may be a significant contribution of direct effects of the viscosity of oat β-glucan on cellular mechanisms of uptake in enterocytes.
URI: http://hdl.handle.net/10214/5323
Date: 2013-01
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