Main content

A Cellular and Molecular Investigation of Dilated Cardiomyopathy (DCM) in Dogs

Show full item record

Title: A Cellular and Molecular Investigation of Dilated Cardiomyopathy (DCM) in Dogs
Author: Sinclair, Elizabeth
Department: Department of Biomedical Sciences
Program: Biomedical Sciences
Advisor: Pyle, Glen
Abstract: We hypothesized that alterations in cardiac myofilaments are associated with hereditary canine DCM. DCM myofilaments demonstrated a reduction in EC50 and a modest decrease in maximum activity compared to non-failing dog samples. Treatment of myofilaments with the calcium sensitizer, bepridil, showed a reduction in EC50. Desmin and tropomyosin phosphorylation was increased in DCM. Desmin protein levels were increased in DCM. Total troponin I phosphorylation was unchanged, but S23/S24 phosphorylation was reduced in DCM. Myofilament-associated PKC-δ and -ζ were elevated in DCM, PKC- ε was modestly reduced, and PKC-α showed no change. These data are the first investigation of cardiac myofilaments in naturally occurring canine DCM, and support the hypothesis that alterations in cardiac myofilaments are associated with DCM.
URI: http://hdl.handle.net/10214/5292
Date: 2012-12


Files in this item

Files Size Format View Description
Sinclair_Elizabeth_201301_Msc.pdf 1.189Mb PDF View/Open thesis

This item appears in the following Collection(s)

Show full item record