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Virulence factors in fecal Escherichia coli from humans and animals

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Title: Virulence factors in fecal Escherichia coli from humans and animals
Author: Hill, Stephen
Department: School of Environmental Sciences
Program: Environmental Sciences
Advisor: Trevors, JackLee, HungEdge, Tom
Abstract: A DNA microarray capable of detecting 445 virulence factors (VFs) and antibiotic resistance genes was used to assess human and animal fecal E. coli isolates for pathogenic potential and host specificity. The only enteric pathotype detected was atypical EPEC, which was found in 3.7% of all isolates. 17% of human isolates were extraintestinal pathotypes, with the majority of these being uropathogenic. Isolates from humans and chickens were the most likely to have resistance to at least one class of antibiotic. VFs that were found almost exclusively in human isolates, when compared to one other group, included sat (10% of human isolates and no animal isolates), iucD and iut (24% of human, <1% mammal) and iha (16% human, <1% wild avian). Decision trees utilizing multiple probes to identify the source of an E. coli isolate were able to correctly identify the source of 79% of validation isolates in a human vs. animal comparison.
Date: 2013-01

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