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Detection of Human Rotavirus in Southern Ontario Source Waters

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dc.contributor.advisor Liss, Steven
dc.contributor.advisor Habash, Marc Davis, Bailey Helena 2013-01-08T16:53:54Z 2013-01-08T16:53:54Z 2012-12 2012-12-03 2013-01-08
dc.description.abstract As part of a larger quantitative microbial risk assessment (QMRA) study, the raw water intakes of 8 different drinking water treatment plants in Ontario were sampled for rotavirus. Group A rotavirus was detected and semi-quantified via RT-qPCR. Rotavirus was detected in 6 of 8 drinking water treatment plant raw water intakes at various sampling times during a 2 year period at estimated quantities of 0 – 513 viral genome copies/L water. As hypothesized, the virus counts showed a seasonal tendency with significant detection most likely to occur during the spring months and a correlation with turbidity measurements. To our knowledge this is the first study exploring the presence of rotavirus in Ontario source waters. With new proposed changes to the Health Canada guidelines regarding the viruses in drinking water, data on the presence of rotavirus in source waters is required for assessment of risk to public health. en_US
dc.description.sponsorship Kingsclear First Nation en_US
dc.language.iso en en_US
dc.subject Human rotavirus en_US
dc.subject RT-qPCR en_US
dc.subject Source water characterization en_US
dc.subject QMRA en_US
dc.title Detection of Human Rotavirus in Southern Ontario Source Waters en_US
dc.type Thesis en_US Environmental Sciences en_US Master of Science en_US School of Environmental Sciences en_US
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