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Regulation of miR-378 in ovarian granulosa cells

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dc.contributor.advisor Li, Julang
dc.contributor.author Baley, Jason
dc.date.accessioned 2013-01-08T14:01:54Z
dc.date.available 2013-01-08T14:01:54Z
dc.date.copyright 2012-12
dc.date.created 2012-12-14
dc.date.issued 2013-01-08
dc.identifier.uri http://hdl.handle.net/10214/5250
dc.description.abstract MicroRNAs (miRNAs) are important gene expression regulators. It has been previously shown that miR-378 down-regulates estradiol production via suppression of aromatase translation. This study examined if the expression of miR-378 is regulated by ovarian factors known to play important roles in reproductive function. Granulosa cells were cultured in various concentrations of ovarian factors, and real-time RT-PCR revealed follicle stimulating hormone (FSH) decreased miR-378. To investigate the mechanism of FSH inhibition, the influence FSH on suppressive transcription factor expression was examined and GATA-4 was found to be significantly increased with FSH. Gain of function and loss of function approaches were used to determine the influence of GATA-4 on miR-378 expression. Our results suggest that down-regulation of miR-378 by FSH is not mediated by the transcriptional repressor, GATA-4. Future studies investigating suppression of transcriptional activators by FSH would be valuable in elucidating the mechanism of miR-378 regulation by FSH. en_US
dc.language.iso en en_US
dc.title Regulation of miR-378 in ovarian granulosa cells en_US
dc.type Thesis en_US
dc.degree.programme Animal and Poultry Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Animal and Poultry Science en_US


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