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Regulation of miR-378 in ovarian granulosa cells

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Title: Regulation of miR-378 in ovarian granulosa cells
Author: Baley, Jason
Department: Department of Animal and Poultry Science
Program: Animal and Poultry Science
Advisor: Li, Julang
Abstract: MicroRNAs (miRNAs) are important gene expression regulators. It has been previously shown that miR-378 down-regulates estradiol production via suppression of aromatase translation. This study examined if the expression of miR-378 is regulated by ovarian factors known to play important roles in reproductive function. Granulosa cells were cultured in various concentrations of ovarian factors, and real-time RT-PCR revealed follicle stimulating hormone (FSH) decreased miR-378. To investigate the mechanism of FSH inhibition, the influence FSH on suppressive transcription factor expression was examined and GATA-4 was found to be significantly increased with FSH. Gain of function and loss of function approaches were used to determine the influence of GATA-4 on miR-378 expression. Our results suggest that down-regulation of miR-378 by FSH is not mediated by the transcriptional repressor, GATA-4. Future studies investigating suppression of transcriptional activators by FSH would be valuable in elucidating the mechanism of miR-378 regulation by FSH.
Date: 2012-12

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