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Response of Day 8 Equine Embryos to Saccharide Solutions at Various Temperatures

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dc.contributor.advisor Chenier, Tracey
dc.contributor.author Foster, Brittany
dc.date.accessioned 2013-01-02T20:11:42Z
dc.date.available 2013-01-02T20:11:42Z
dc.date.copyright 2012-12
dc.date.created 2012-12-12
dc.date.issued 2013-01-02
dc.identifier.uri http://hdl.handle.net/10214/5136
dc.description.abstract The response of Day Eight equine embryos to saccharide solutions was investigated as a first step in their potential use as non-permeating cryoprotective agents. Embryos were exposured to seven increasing concentrations of either sucrose or galactose at a temperature of 22°, 30° or 37°C. Each embryo was then rehydrated by exposing it to the same solutions in reverse. Embryos underwent osmotic dehydration, independent of treatment group, but embryo size had a significant effect on the response pattern. Embryos < 500µm dehydrated osmotically to 20% of their original volume. Those > 500µm exhibited a delayed response and only dehydrated to 40% of their original volume. When placed into decreasing concentrations, embryos partially rehydrated. Pre- and post-treatment embryo quality was compared and embryos were stained to determine the amount of apoptosis, with no difference in embryo survival between treatments. Results indicate that saccharides show promise for use in equine embryos < 500µm. en_US
dc.description.sponsorship Ontario Veterinary College, Equine Guelph, Partnar Animal Health en_US
dc.language.iso en en_US
dc.subject Horse en_US
dc.subject Equine en_US
dc.subject Reproduction en_US
dc.subject Reproductive Technology en_US
dc.subject Cryopreservation en_US
dc.subject Non-permeating cryoprotecant en_US
dc.subject Osmotic Response en_US
dc.subject Dehydration en_US
dc.subject Saccharides en_US
dc.subject Temperature effect en_US
dc.title Response of Day 8 Equine Embryos to Saccharide Solutions at Various Temperatures en_US
dc.type Thesis en_US
dc.degree.programme Population Medicine en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Population Medicine en_US
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