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Developmental Plasticity of the Cellular Hypoxia Response in Zebrafish, Danio rerio

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dc.contributor.advisor Bernier, Nicholas
dc.contributor.advisor Wright, Patricia Robertson, Cayleih 2012-12-05T14:40:45Z 2012-12-05T14:40:45Z 2012-12 2012-11-30 2012-12-05
dc.description.abstract In most organisms the cellular response to hypoxia is mediated by the master regulator hypoxia-inducible factor-1 (HIF-1). Zebrafish embryos can also arrest development (suspended animation) to tolerate low oxygen. I tested the hypothesis that induction of HIF-1 and associated target genes (eg. erythropoietin) during embryonic development would alter the hypoxia tolerance phenotype of larval and adult fish. I exposed zebrafish embryos at 3 developmental stages to acute (4 h) bouts of hypoxia (5% dissolved oxygen, DO) or anoxia (<0.5% DO). I found that embryos that mount a HIF-1 response have a greater hypoxia tolerance as larvae. Additionally, populations that experienced embryonic HIF-1 induction show an increase in the proportion of males (~70% male), that are more hypoxia tolerant than female fish, compared to control populations (~45% male). Overall, induction of HIF-1 during ontogeny alters the larval and adult zebrafish phenotype to better tolerate future hypoxic bouts. en_US
dc.description.sponsorship NSERC en_US
dc.language.iso en en_US
dc.rights.uri *
dc.subject Developmental Plasticity en_US
dc.subject HIF-1 en_US
dc.subject Hypoxia en_US
dc.subject Zebrafish en_US
dc.title Developmental Plasticity of the Cellular Hypoxia Response in Zebrafish, Danio rerio en_US
dc.type Thesis en_US Integrative Biology en_US Master of Science en_US Department of Integrative Biology en_US
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