Title:
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Developmental Plasticity of the Cellular Hypoxia Response in Zebrafish, Danio rerio |
Author:
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Robertson, Cayleih
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Department:
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Department of Integrative Biology |
Program:
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Integrative Biology |
Advisor:
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Bernier, Nicholas Wright, Patricia |
Abstract:
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In most organisms the cellular response to hypoxia is mediated by the master regulator hypoxia-inducible factor-1 (HIF-1). Zebrafish embryos can also arrest development (suspended animation) to tolerate low oxygen. I tested the hypothesis that induction of HIF-1 and associated target genes (eg. erythropoietin) during embryonic development would alter the hypoxia tolerance phenotype of larval and adult fish. I exposed zebrafish embryos at 3 developmental stages to acute (4 h) bouts of hypoxia (5% dissolved oxygen, DO) or anoxia (<0.5% DO). I found that embryos that mount a HIF-1 response have a greater hypoxia tolerance as larvae. Additionally, populations that experienced embryonic HIF-1 induction show an increase in the proportion of males (~70% male), that are more hypoxia tolerant than female fish, compared to control populations (~45% male). Overall, induction of HIF-1 during ontogeny alters the larval and adult zebrafish phenotype to better tolerate future hypoxic bouts. |
URI:
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http://hdl.handle.net/10214/4749
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Date:
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2012-12 |
Terms of Use:
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