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Identification and characterization of tail-anchored (TA) membrane proteins within the Arabidopsis proteome, and characterization of the subcellular sorting signals of plastid-localized TA proteins

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Title: Identification and characterization of tail-anchored (TA) membrane proteins within the Arabidopsis proteome, and characterization of the subcellular sorting signals of plastid-localized TA proteins
Author: Dhanoa, Preetinder
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Mullen, Robert
Abstract: Tail-anchored (TA) membrane proteins are a class of proteins characterized by their lack of N-terminal signal sequence which play critical roles in apoptosis, vesicle trafficking and other vital processes in eukaryotic organisms. Until recently, this class of membrane proteins has been relatively unknown in plants. This thesis presents the results of bioinformatic analysis that identifies a large group of putative candidate TA proteins within the Arabidopsis proteome, and begins characterizing several newly-identified plant TA proteins. Also presented here are the results from a comprehensive study aimed at investigating the biogenesis of three plastid TA proteins, including the 33-kDa and 34-kDa GTPases of the translocon at the outer envelope of chloroplasts (Toc33 and Toc34) and a novel 9-kDa protein of unknown function that we define here as an outer envelope TA protein (OEP9). Results presented here provide evidence in support of at least two sorting pathways for plastid TA outer envelope proteins and shed light on not only the complex diversity of pathways involved in the targeting and insertion of proteins into plastids, but also the molecular mechanisms that underlie the delivery of TA proteins to their proper intracellular locations in general.
URI: http://hdl.handle.net/10214/4062
Date: 2012-09


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