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VHH Antibody Fragments Against Internalin B, a Virulence Factor of Listeria monocytogenes: Reagents for Biosensor Development

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dc.contributor.advisor Hall, J. Christopher
dc.contributor.advisor Mackenzie, C. Roger
dc.contributor.author Gene, Robert
dc.date.accessioned 2012-10-04T15:03:47Z
dc.date.available 2012-10-04T15:03:47Z
dc.date.copyright 2012-09
dc.date.created 2012-09-28
dc.date.issued 2012-10-04
dc.identifier.uri http://hdl.handle.net/10214/4051
dc.description.abstract The food processing industry requires alternative methods for detecting the foodborne pathogen Listeria monocytogenes that are cheaper and faster than the current methods. Conventional antibodies and their fragments have been used as biorecognition elements in sensors before, but their use is hindered by high production cost and relative instability. These issues are resolved by VHH fragments, derived from the heavy chain-only antibodies found in Camelidae. VHHs are inexpensive to produce, and are more resistant to environmental stressors. This work describes the isolation of phage-displayed VHHs that recognize recombinant Internalin B, a virulence factor characteristic of L. monocytogenes. Clone R303 was chosen for further characterization, and shown to bind full-length Internalin B. Furthermore, immobilized R303 was shown to capture L. monocytogenes cells. This panel of VHHs, particularly R303, can be utilized by colleagues within the Sentinel Bioactive Paper Network to make a viable biosensor for L. monocytogenes. en_US
dc.description.sponsorship Sentinel Bioactive Paper Network en_US
dc.language.iso en en_US
dc.subject Antibody en_US
dc.subject VHH en_US
dc.subject Listeria monocytogenes en_US
dc.subject Bioactive paper en_US
dc.subject Biacore en_US
dc.subject Biosensor en_US
dc.title VHH Antibody Fragments Against Internalin B, a Virulence Factor of Listeria monocytogenes: Reagents for Biosensor Development en_US
dc.type Thesis en_US
dc.degree.programme Environmental Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Environmental Biology en_US
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