Main content

Characterization of CGI58 and PXA1 in Plant Lipolysis

Show full item record

Title: Characterization of CGI58 and PXA1 in Plant Lipolysis
Author: Khuu, Nicholas
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Mullen, Robert
Abstract: CGI58 (Comparative Gene Identifier 58) is considered a key regulator of lipolysis since the disruption of CGI58 in Arabidopsis results in the hyper-accumulation of lipid droplets in vegetative tissues. However, the molecular mechanism underlying this process has not been well studied. To address this, Arabidopsis lines stably expressing RNAi against transcripts encoding one or both of the CGI58 isoforms were generated and, using RT-PCR, specific alterations in CGI58 gene expression were shown to be achieved. Additionally, split-ubiquitin two-hybrid and bimolecular fluorescence complementation assays indicated that CGI58 interacts, albeit weakly, with PXA1, a peroxisomal transport protein responsible for the uptake of various lipid metabolites. Finally, results from mutational analyses of PXA1 revealed that the protein’s peroxisomal membrane targeting information is located within its N-terminal 180 amino acids. Taken together, these results have important implications for understanding the coordinated roles of CGI-58 and PXA1 in regulating lipid homeostasis in plants.
URI: http://hdl.handle.net/10214/3985
Date: 2012-09
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Khuu_Nicholas_201209_Msc.pdf 3.735Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record

http://creativecommons.org/licenses/by-nd/2.5/ca/ Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nd/2.5/ca/