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Comparing influenza virus hemagglutinin (HA) expression in three different baculovirus expression systems

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Title: Comparing influenza virus hemagglutinin (HA) expression in three different baculovirus expression systems
Author: Elliott, Alexandra
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Krell, Peter J
Abstract: In this study, the expression of HA, a key immunogenic protein of influenza viruses, in insect cells was compared using three baculovirus expression strategies: protein over-expression, surface (GP64) display, and capsid (VP39) display. Further, a recombinant virus expressing NA, another immunogenic influenza virus protein, was generated and fused to an HA epitope-tag. Western immunoblot using various antibodies, including those against HA, demonstrated the expression of HA and NA for all recombinant viruses. HA showed stronger expression when fused to the C-terminus of VP39 than the N-terminus, but unlike other expression methods, there was no observable cleavage of HA in VP39-displayed viruses. Cells infected with only over-expressed and surfaced-displayed HA were biologically active, and capable of hemadsorption and hemagglutination of chicken red blood cells. These results suggest that GP64 display or over-expression are the most efficacious modes of HA-expression for use as antigen to detect anti-HA antibodies in poultry.
URI: http://hdl.handle.net/10214/3916
Date: 2012-08


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