Main content

Angiogenesis During Multi-tissue Regeneration Following Tail Loss in the Leopard Gecko (Eublepharis macularius)

Show full item record

Title: Angiogenesis During Multi-tissue Regeneration Following Tail Loss in the Leopard Gecko (Eublepharis macularius)
Author: Payne, Samantha Louise
Department: Department of Biomedical Sciences
Program: Biomedical Sciences
Advisor: Vickaryous, Matthew
Abstract: Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is important in post-injury scar formation but its role in scar-free regeneration remains relatively unexplored. This study investigates vascular regeneration during tail regeneration in the leopard gecko (Eublepharis macularius). It is hypothesized that blood vessel regeneration follows a conserved sequence of events similar to physiological angiogenesis. To test this hypothesis the onset and pattern of expression of common vascular and angiogenic proteins (von Willebrand factor, α-smooth muscle actin, vascular endothelial growth factor, thrombospondin-1 and cluster differentiation 36) was investigated. The effect of the anti-angiogenic peptide ABT-510 on tail regeneration was also explored by documenting changes in vascular morphology and histology of regenerate tails. Results show that the proteins of interest are expressed in a conserved sequence consistent with physiological angiogenesis. ABT-510 did not consistently prevent tail regeneration, but did have some small-scale effects. These results provide the basis for further investigations into the importance of angiogenesis during multi-tissue regeneration.
URI: http://hdl.handle.net/10214/3900
Date: 2012-08


Files in this item

Files Size Format View
S Payne Thesis.pdf 2.974Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record