Main content

Evaluation of Genes Encoding the Enzymes of the Kennedy Pathway in Soybeans with Altered Fatty Acid Profiles

Show simple item record

dc.contributor.advisor Rajcan, Istvan McNaughton, Amy J. M. 2012-06-28T19:26:57Z 2012-06-28T19:26:57Z 2012-06 2012-06-08 2012-06-28
dc.description.abstract Soybean (Glycine max (L.) Merr) is the largest oil and protein crop in the world and it is grown for both oil and protein. To address the needs of both the edible oil market and industrial applications of soybean oil, fatty acid modification has been a focus of soybean breeding programs. Natural variation, mutagenesis and genetic engineering have been used to alter the fatty acid profile. Several genes, mostly desaturases, have been associated with altered fatty acid profiles but enzymes in the Kennedy Pathway have yet to be studied as another source of genetic variation for altering the fatty acid profiles. The Kennedy Pathway is also known as the oil producing pathway and consists of four enzymes: glycerol-3-phosphate acyltransferase (G3PAT); lysophosphatidic acid acyltransferase (LPAAT); phosphatidic acid phosphatase (PAP); and diacylglycerol acyltransferase 1 (DGAT1). The starting material for this pathway is glycerol-3-phosphate, which is produced from glycerol by glycerol kinase (GK), and the product of this pathway is triacylglycerol (TAG). The overall objective of this study was to elucidate the role that the Kennedy Pathway plays in determining the fatty acid profile in two ways: (1) sequencing the transcribed region of the genomic genes encoding the enzymes of GK, G3PAT, LPAAT, and DGAT1 in soybean genotypes with altered fatty acid profiles; and (2) studying their expression over seed development, across three growing temperatures. The genetic material for the study consisted of four soybean genotypes with altered fatty acid profile: RG2, RG7, RG10, and SV64-53. Results from sequencing showed that the mutations identified in G3PAT, LPAAT, and DGAT1 in the four soybean genotypes did not explain the differences in the fatty acid profiles. The expression of G3PAT, LPAAT, and DGAT1 over seed development showed that G3PAT had the lowest levels, followed by LPAAT, then DGAT1, across the growing temperatures. The differences in expression among genotypes corresponded to differences in fatty acid accumulation, suggesting that expression rather than genetic mutations in the transcribed region of the genes influenced the fatty acid profile of the genotypes in this study. In conclusion, the enzymes of the Kennedy Pathway appear to contribute to the altered fatty acid profiles observed in the soybean mutant genotypes. en_US
dc.description.sponsorship Ontario Ministry of Economic Development and Innovation (formerly Ontario Ministry of Research and Innovation), BioCar Initiative, Grain Farmers of Ontario, SeCan en_US
dc.language.iso en en_US
dc.subject fatty acids en_US
dc.subject soybean en_US
dc.subject Kennedy Pathway en_US
dc.subject glycerol-3-phosphate acyltransferase (G3PAT) en_US
dc.subject lysophosphatidic acid acyltransferase (LPAAT) en_US
dc.subject diacylglycerol acyltransferase 1 (DGAT1) en_US
dc.subject glycerol kinase (GK) en_US
dc.subject triacylglycerol (TAG) en_US
dc.title Evaluation of Genes Encoding the Enzymes of the Kennedy Pathway in Soybeans with Altered Fatty Acid Profiles en_US
dc.type Thesis en_US Plant Agriculture en_US Doctor of Philosophy en_US Department of Plant Agriculture en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.

Files in this item

Files Size Format View
full_thesis_-_final_submission.pdf 1023.Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record