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A MULTIGENE APPROACH FOR INVESTIGATING DNA BARCODE LINEAGES IN PROVISIONAL CRYPTIC SPECIES OF LEPIDOPTERA IN COSTA RICA

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dc.contributor.advisor Hajibabaei, Mehrdad
dc.contributor.author Bertrand, Claudia
dc.date.accessioned 2012-05-04T14:37:44Z
dc.date.available 2012-05-04T14:37:44Z
dc.date.copyright 2012-03
dc.date.created 2012-03-23
dc.date.issued 2012-05-04
dc.identifier.uri http://hdl.handle.net/10214/3560
dc.description.abstract DNA barcoding has illuminated genetically distinct lineages within what appears to be one morphological species. For example, a large-scale DNA barcode analysis of Lepidoptera in the Área de Conservación Guanacaste has revealed that 8% of the morphospecies show more than one DNA barcode lineage. To assess the evolutionary significance of five of these lineages I conducted further molecular analyses by sequencing mitochondrial cytochrome b, nuclear Elongation Factor 1α and ribosomal Internal Transcribed Spacer 2, and compare their gene phylogenies with the provisional species tree hypothesized by DNA barcode genetic distances. Both mitochondrial and nuclear markers support the existence of three cryptic species in one of the five cases. Morphological and ecological correlates are still lacking to understand the origin of this divergence. The lack of corroboration between markers in the four remaining species either suggests that the chosen nuclear markers have not diverged since speciation, or there has been recent hybridization between lineages. In one case, hybridization is strongly suggested. en_US
dc.description.sponsorship Natural Sciences and Engineering Research Council of Canada, Genome Canada through the Ontario Genomics Institute en_US
dc.language.iso en en_US
dc.subject DNA barcoding en_US
dc.subject Lepidoptera en_US
dc.subject Cryptic species en_US
dc.title A MULTIGENE APPROACH FOR INVESTIGATING DNA BARCODE LINEAGES IN PROVISIONAL CRYPTIC SPECIES OF LEPIDOPTERA IN COSTA RICA en_US
dc.type Thesis en_US
dc.degree.programme Integrative Biology en_US
dc.degree.name Master of Applied Science en_US
dc.degree.department Department of Integrative Biology en_US
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