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Arabidopsis glyoxylate reductase 1 is localized in the cytosol and not peroxisomes in plant cells

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dc.contributor.advisor Mullen, Robert T Ching, Steven LK 2012-02-13T14:48:26Z 2012-02-13T14:48:26Z 2012-02 2012-02-01 2012-02-01
dc.identifier.citation Ching SLK, Gidda SK, Rochon A, van Cauwenberghe OR, Shelp BJ, Mullen RT 2012 Glyoxylate Reductase (GLYR1) is localized to the cytosol not peroxisomes in plant cells, J. Integr. Plant Biol. en_US
dc.description.abstract Glyoxylate reductase (GLYR) is a key enzyme in plant metabolism which catalyzes the detoxification of both photorespiratory glyoxylate and succinic semialdehdye, an intermediate of the γ-aminobutyrate (GABA) pathway. Two isoforms of GLYR exist in plants, GLYR1 and GLYR2, and while GLYR2 is known to be localized in plastids, GLYR1 has been reported to be localized in either peroxisomes or the cytosol. Here, the intracellular localization of Arabidopsis GLYR1 was reappraised by conducting microscopy-based experiments that address some novel mechanisms by which proteins can be directed to peroxisomes. For instance, the C-terminal tripeptide sequence of GLYR1, -SRE, despite its resemblance to a type 1 peroxisomal targeting signal, was not sufficient for peroxisomal targeting. Collectively, the results define the cytosol as the intracellular location of GLYR1 and provide a useful reference for future studies of proteins proposed to be localized to peroxisomes and/or the cytosol. en_US
dc.description.sponsorship NSERC en_US
dc.language.iso en en_US
dc.publisher Journal of Intergrative Plant Biology en_US
dc.rights.uri *
dc.subject Arabidopsis en_US
dc.subject GABA en_US
dc.subject GLYR1 en_US
dc.subject cytosol en_US
dc.subject photobleaching en_US
dc.subject peroxisome en_US
dc.subject PTS en_US
dc.subject stress en_US
dc.subject tobacco BY-2 en_US
dc.title Arabidopsis glyoxylate reductase 1 is localized in the cytosol and not peroxisomes in plant cells en_US
dc.type Thesis en_US Molecular and Cellular Biology en_US Master of Science en_US Department of Molecular and Cellular Biology en_US
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