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Utilization of Biodiesel-Derived Crude Glycerol by Fungi for Biomass and Lipid Production

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Title: Utilization of Biodiesel-Derived Crude Glycerol by Fungi for Biomass and Lipid Production
Author: Marchand, Kimberly
Department: Department of Land Resource Science
Program: Land Resource Science
Advisor: Nicol, Robert
Abstract: Rapid expansion of the biodiesel industry has led to a surplus of crude glycerol. This thesis investigates the ability of fungi to utilize crude glycerol as an alternative to conventional carbon substrates for growth and lipid production. Screening revealed that 40 of the 61 isolates tested had increased biomass yield, compared to glucose, when crude glycerol was utilized; 29 of these isolates possessed the ability to completely metabolize 14 g•L-1 of glycerol after 7-14 days. The top four candidates belonged to the genera Galactomyces and Mucor. Overall, Galactomyces sp. proved to be better suited for lipid production. In addition to producing biomass with a high lipid content (up to 45 % w/w), Galactomyces sp. also exhibited high biomass yields (up to 25 g•L-1). The results obtained in this study compare favourably, and in some cases exceed, other literature reported values for biomass and lipid production using glycerol.
Date: 2012-01
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