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CHARACTERIZATION OF SlMCA2, A NOVEL TYPE I METACASPASE IN SOLANUM LYCOPERSICUM AND ITS IMPLICATIONS IN PROGRAMMED CELL DEATH

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dc.contributor.advisor Greenwood, John
dc.contributor.author Suvajac, Ema
dc.date.accessioned 2011-12-16T19:09:07Z
dc.date.available 2011-12-16T19:09:07Z
dc.date.copyright 2011-12
dc.date.created 2011-12-07
dc.date.issued 2011-12-16
dc.identifier.uri http://hdl.handle.net/10214/3197
dc.description.abstract Programmed cell death (PCD) is an indispensible process in plant and animal systems that serves to eliminate cells and/or tissues and recycle nutrients from these tissues to the rest of the organism. In animals, PCD is referred to as apoptosis and is performed by caspases, a family of aspartate-specific cysteine proteinases that serve to perceive the cell death signal and execute the cell death phenotype. In 2000, Uren et al. discovered a new family of cysteine proteinases in plants called metacaspases – distant arginine/lysine-specific relatives of animal caspases – thought to be involved in plant PCD. The goal of this study was to correlate SlMCA2 expression with PCD in tomato. Polyclonal antibodies were obtained against the Type I metacaspase SlMCA2 in Solanum lycopersicum and used for Western blot analyses. BY-2 cell biolistics and in-situ hybridization were used to investigate where SlMCA2 protein and mRNA accumulate in various tissues. Results produced were replicated a minimum of three times and correlate SlMCA2 to PCD, but not initiation of PCD. en_US
dc.language.iso en en_US
dc.subject programmed cell death tomato plant type I metacaspase SLMCA2 en_US
dc.title CHARACTERIZATION OF SlMCA2, A NOVEL TYPE I METACASPASE IN SOLANUM LYCOPERSICUM AND ITS IMPLICATIONS IN PROGRAMMED CELL DEATH en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US


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