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Investigating a role for Fusobacterium nucleatum in Inflammatory Bowel Disease

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dc.contributor.advisor Allen-Vercoe, Emma
dc.contributor.author Strauss, Jaclyn
dc.date.accessioned 2011-09-08T18:14:03Z
dc.date.available 2011-09-08T18:14:03Z
dc.date.copyright 2011-09
dc.date.created 2011-08-30
dc.date.issued 2011-09-08
dc.identifier.uri http://hdl.handle.net/10214/2965
dc.description.abstract Inflammatory Bowel Disease (IBD) is an umbrella term used to describe a group of chronic, relapsing/remitting disorders of the gastrointestinal tract (GIT). While the precise aetiology of IBD is unknown, it is believed to be a result of the interaction of genetics, the immune system and the enteric microbiota. Thus, the search for potentially pathogenic microbial residents of the GIT is a current research focus. Fusobacterium nucleatum (Fn) is a member of the normal human microflora, including the GIT and has a well-characterized role in periodontitis in the oral setting. We have determined that Fn can be frequently recovered from human intestinal biopsies and furthermore, there is a positive correlation between recovery of Fn and the IBD status of the host. Fn strains from IBD patients were more invasive in vitro than strains from healthy controls and also demonstrated the ability to survive and proliferate inside host cells. Furthermore, while Fn strains from both IBD patients and healthy controls were able to induce expression of the pro-inflammatory cytokine IL-8 in vitro, in comparison to strains from controls, Fn strains from IBD patients resulted in decreased levels of IL-8 protein outside the host cells, suggesting that these strains may utilize sophisticated tactics to promote their survival. Thus, differences in virulence determinants among strains may be key to understanding a potential role for Fn in IBD. Characterization of virulence mechanisms utilized by Fn isolates from IBD patients could define a potentially important aspect of microbe/host interactions in this devastating disease, and indicate future therapeutic targets. en_US
dc.description.sponsorship Canadian Institutes of Health Research, Canadian Digestive Health Foundation, Crohn's and Colitis Foundation of Canada en_US
dc.language.iso en en_US
dc.subject Fusobacterium nucleatum en_US
dc.subject Inflammatory Bowel Disease en_US
dc.title Investigating a role for Fusobacterium nucleatum in Inflammatory Bowel Disease en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Doctor of Philosophy en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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