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INVESTIGATION OF AXIN2 IN ZEBRAFISH (DANIO RERIO) DEVELOPMENT AND ITS ROLE IN CANONICAL WNT SIGNALING

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Title: INVESTIGATION OF AXIN2 IN ZEBRAFISH (DANIO RERIO) DEVELOPMENT AND ITS ROLE IN CANONICAL WNT SIGNALING
Author: Lum, Whitney
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Van Raay, Terry
Abstract: Canonical Wnt signaling is involved in many aspects of development including axis specification and anterior-posterior neuroectoderm formation during vertebrate embryogenesis. Axin2, a homologue of Axin1, is thought to have a similar regulatory role within the cell, but differences in their expression and binding partners suggest Axin2 is not completely redundant with Axin1. To better understand Axin2 in canonical Wnt signaling, I utilized several approaches to explore its expression and function. In the zebrafish embryo, I found Axin2 is expressed in known active domains of Wnt signaling, suggesting an inducible regulatory role. Additionally, canonical Wnt signaling was sufficient and necessary to induce Axin2 expression and Axin2 was sufficient and necessary to inhibit Wnt signaling. As Wnt signaling is important in development and its dysregulation has been implicated in diseases such as colorectal cancer, this study helps advance our understanding of how Wnt signaling regulates itself through the use of negative feedback inhibitors, such as Axin2.
URI: http://hdl.handle.net/10214/2876
Date: 2011-08


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