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The effects of delta-9 tetrahydrocannabinol (THC) on granulosa cell viability, apoptosis, and gene expression

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dc.contributor.advisor Favetta, Laura Dufour, Jaustin 2022-08-05T16:53:46Z 2022-08-05T16:53:46Z 2022-07 2022-07-28
dc.description.abstract This thesis investigates the effects of the psychoactive component of cannabis, delta-9 tetrahydrocannabinol (THC) on bovine in vitro granulosa cell viability, apoptosis, and stress response pathway. 11βHSD1-2 convert cortisol into active and inactive forms. Upon cortisol activation, the glucocorticoid receptor (GR) translocates to the nucleus to regulate transcription. MAPK8-9 enhance GR nuclear export and terminate GR-mediated transcription. Heat shock protein 70 and 90 (HSP70, HSP90) are involved in both THC and cortisol pathways. This study hypothesized that THC counteracts cortisol’s effects by modulating 11βHSDs, increasing GR nuclear export and oversaturating HSPs. Cell viability and apoptosis was assessed in response to THC at clinically relevant doses. Gene expression was analyzed in response to THC and cortisol treatments during culture. The experimental data revealed a potential ability for THC to reduce cortisol signalling in GCs. No effect of THC on cell viability and apoptosis was revealed. en_US
dc.language.iso en en_US
dc.publisher University of Guelph en_US
dc.subject stress en_US
dc.subject cannabis en_US
dc.subject thc en_US
dc.subject fertility en_US
dc.subject granulosa cell en_US
dc.title The effects of delta-9 tetrahydrocannabinol (THC) on granulosa cell viability, apoptosis, and gene expression en_US
dc.type Thesis en_US Biomedical Sciences en_US Master of Science en_US Department of Biomedical Sciences en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated. University of Guelph en

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