Main content

Azoreduction: Reductive Metabolism of Azo Food Dyes by Species of the Human Gut Microbiome

Show full item record

Title: Azoreduction: Reductive Metabolism of Azo Food Dyes by Species of the Human Gut Microbiome
Author: Elder, Riley
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Allen-Vercoe, EmmaJosephy, David
Abstract: Azo dye food colourants are widely used in Canada and other countries. When ingested, these dyes may be reduced by azoreductases of the gut microbiome bacteria to metabolites that might have immune-, neuro-, or genotoxic effects. I have identified obligate anaerobic bacterial species from the human gut that reduce these azo food dyes. Bacterial species representing six phyla were derived from human fecal microbiomes. Bacteria were incubated on dye-infused plates and decolourization was monitored by visual inspection. Taxa exhibiting high azoreductase activity belonged to the genera Clostridium, Hungatella, Enterocloster, Veillonella, Dielma, Eggerthella, Odoribacter, and Phocaeicola. The effects of the dyes on bacterial growth were studied. The genome sequences of two Veillonella spp. with high azo dye reduction activities were examined. Candidate genes were cloned and expressed as recombinant proteins, but none showed detectable azo dye reduction activity in vitro.
URI: https://hdl.handle.net/10214/26997
Date: 2022-05-20
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Elder_Riley_202205_MSc.pdf 2.081Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record