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Regulation of membrane phospholipid balance under conditions of impaired CDP-ethanolamine pathway

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Title: Regulation of membrane phospholipid balance under conditions of impaired CDP-ethanolamine pathway
Author: Iraji, Roya
Department: Department of Human Health and Nutritional Sciences
Program: Human Health and Nutritional Sciences
Advisor: Bakovic, Marica
Abstract: Phosphatidylethanolamine (PE) is produced by the CDP-ethanolamine Kennedy pathway and by phosphatidylserine (PS) decarboxylation. We explored the role of PS synthesis from phosphatidylcholine (PC) and PE by PS-synthase 1 (PSS1) and PS-synthase 2 (PSS2) base-exchange reactions as well as the role of PS decarboxylation by PISD in providing PE when its synthesis is reduced by downregulation of Pcyt2. Results demonstrate that in Pcyt2 deficient human fibroblasts, choline and ethanolamine transport is increased by upregulation of CTL1. Choline treatment intensified this transport by upregulation of CTL2. Increased choline increased PC pool but it did not stimulate the PC or PE exchange to PS by PSS1 or PSS2. Although the PS pool increased, the PS decarboxylation to PE did not compensate for the reduced PE synthesis by the CDP-ethanolamine pathway. The total PE pool was mainly preserved by reduced degradation of PE, synthesized by the remained activity of the CDP-ethanolamine pathway.
URI: https://hdl.handle.net/10214/26970
Date: 2022-05
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