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Investigating the Proteomic Responses of Gram-Negative and Gram-Positive Bacteria to a Novel Non-Traditional Silver-Based Antimicrobial

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Title: Investigating the Proteomic Responses of Gram-Negative and Gram-Positive Bacteria to a Novel Non-Traditional Silver-Based Antimicrobial
Author: Goodall, Carlie
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Khursigara, Cezar
Abstract: Recently, the World Health Organization (WHO) has declared a global shortage of innovative antimicrobials that address antimicrobial resistance and emphasized the devastating toll antimicrobial resistance is taking on our healthcare systems. Due to their proposed multi-targeted mechanisms and lower incidence of resistance, silver-based compounds are ideal candidates for further research and development. To investigate the utility of a newly developed silver-based antimicrobial, EPC-373K, against common wound pathogens we employed antimicrobial susceptibility and LFQ proteomic techniques. Exploration of the global proteomic shifts and identification of several pathways and processes disrupted in Pseudomonas aeruginosa PAO1 and Staphylococcus aureus TCH959 has furthered our understanding of the mechanism(s) employed by EPC-373K. Distinct profiles characterized by unique physiological changes were observed for each strain highlighting differences in the responses of Gram-negative and Gram-positive species to EPC-373K. Characterization of the impact of EPC-373K on wound pathogens assists in defining the utility of this novel antimicrobial within the current field of therapies targeting topical infections.
URI: https://hdl.handle.net/10214/26949
Date: 2022-05
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