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The Effect of Interleukin-10 Receptor Alpha on Bovine Mammary Epithelial Cells Infected with Mycobacterium avium subsp. paratuberculosis.

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dc.contributor.advisor Baes, Christine
dc.contributor.author Fong, Aisha
dc.date.accessioned 2022-01-13T19:26:27Z
dc.date.available 2022-01-13T19:26:27Z
dc.date.copyright 2022-01
dc.date.created 2021-12-22
dc.identifier.uri https://hdl.handle.net/10214/26682
dc.description.abstract Johne’s disease is a chronic wasting disease in dairy cattle caused by the bacterium Mycobacterium avium subspecies paratuberculosis (MAP). Genetic selection can help to control this disease, and one candidate gene of interest is the gene encoding for interleukin-10 receptor subunit alpha (IL10Ra). A bovine mammary epithelial cell line was created with the gene encoding IL10Rα knocked out (IL10KO). This cell line was exposed to live MAP, and mRNA was extracted. The differentially expressed genes were compared between the IL10KO and wild-type cell lines, and gene ontology was performed. There was a difference in immune response between the IL10KO and wild-type cells, and little difference between the uninfected IL10KO cells and the infected IL10KO cells. Haplotypes 0.5Mb upstream and downstream of IL10Ra associated with a positive test for Johne’s disease were not found within a population of 618 cows, however, there was genetic variation, meaning there is potential for selection. en_US
dc.language.iso en en_US
dc.publisher University of Guelph en
dc.rights Attribution 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ *
dc.subject IL10RA en_US
dc.subject Dairy Cattle en_US
dc.subject Genetics en_US
dc.subject Immunology en_US
dc.subject Johne's Disease en_US
dc.title The Effect of Interleukin-10 Receptor Alpha on Bovine Mammary Epithelial Cells Infected with Mycobacterium avium subsp. paratuberculosis. en_US
dc.type Thesis en_US
dc.degree.programme Animal and Poultry Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Animal Biosciences en_US
dc.degree.grantor University of Guelph en


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