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Investigating the Hypoxia-Induced Alternative Splicing Event of Eukaryotic Ribosomal Protein S24

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Title: Investigating the Hypoxia-Induced Alternative Splicing Event of Eukaryotic Ribosomal Protein S24
Author: Kerry, Jenna
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Uniacke, James
Abstract: In response to hypoxia, eukaryotic cells have developed mechanisms that control gene expression in order to survive. Given that hypoxia is a characteristic of the tumour microenvironment, hypoxic stress response mechanisms provide tumour cells with survival benefits, thereby increasing oncogenic potential. One mechanism, that has been referred to as the 11th hallmark of cancer, is hypoxia-induced alternative splicing. Research has shown that hypoxia changes expression of splicing regulators which, as a result, changes alternative splicing patterns of cancer-associated genes and promotes metastatic disease. Here, we investigate the alterative splicing of ribosomal protein S24 in human glioblastoma cells using qPCR after exposure to hypoxia and/or a plethora of drug treatments. Here, we show data that suggests that an alternative splicing in RPS24 is, in part, due to the hypoxia inducible factor response mechanism and possibly induction of autophagy, and that this response aids in survival during hypoxic stress.
URI: https://hdl.handle.net/10214/26673
Date: 2022-01
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Embargoed Until: 2022-12-01


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