Main content

Effect of Proteasome Inhibitors on Canine Lymphoma Cell Response to CHOP Chemotherapy In Vitro

Show full item record

Title: Effect of Proteasome Inhibitors on Canine Lymphoma Cell Response to CHOP Chemotherapy In Vitro
Author: Prevedel, Nicholas Ennio
Department: Department of Biomedical Sciences
Program: Biomedical Sciences
Advisor: Wood, GeoffreyCoomber, Brenda
Abstract: Canine lymphoma is commonly treated with CHOP chemotherapy. Proteasome inhibitors are employed with CHOP for the treatment of human hematological malignancies. The aim of this study was to determine the effect of proteasome inhibitors on canine B-cell lymphoma cell line (CLBL-1) cell viability, and to investigate whether proteasome inhibitors sensitize cells to CHOP agents. We hypothesized that proteasome inhibitors will reduce CLBL-1 viability and will be synergistic with CHOP compounds. CLBL-1 was sensitive to proteasome inhibition. The IC50 of bortezomib was 15.1 nM and of ixazomib was 59.14 nM. Proteasome inhibitors plus doxorubicin had a synergistic effect on CLBL-1 viability, with vincristine there were different effects depending on the combination ratio, and there was an antagonistic effect with 4-HC (cyclophosphamide). These results have clinical utility, as proteasome inhibition could potentially be used with a synergizing CHOP compound to improve responsiveness to chemotherapy for canine lymphoma patients.
URI: https://hdl.handle.net/10214/26654
Date: 2021-12
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Prevedel_Nicholas_202112_MSc.pdf 1.121Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record