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Ablation of CKMT1 Does Not Affect Energy Expenditure, Adipose Tissue Mitochondrial Respiration or Susceptibility to High-fat Diet-induced Obesity

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dc.contributor.advisor Holloway, Graham
dc.contributor.author Politis-Barber, Valerie
dc.date.accessioned 2021-08-31T19:56:22Z
dc.date.copyright 2021-07
dc.date.created 2021-07-22
dc.identifier.uri https://hdl.handle.net/10214/26331
dc.description.abstract Thermogenic adipose tissues are studied as a prospective anti-obesity target for their ability to increase energy expenditure (EE) by dissipating chemical energy through non-shivering thermogenesis (NST). A novel effector of NST has been identified in vitro, whereby creatine stimulates mitochondrial respiration when ADP is limiting. The mechanism is proposed to be mediated in part by a creatine kinase enzyme, however the specific isoform remains elusive. Since creatine kinase ubiquitous-type, mitochondrial (CKMT1) is a candidate, we utilized Ckmt1 knockout (KO) mice to assess its biological contribution to EE and mitochondrial respiration in iWAT and gWAT. Interestingly, we could not recapitulate the stimulatory effects of creatine ex vivo, and Ckmt1 KO mice did not display altered responses to pharmacological adrenergic activation or a high-fat diet. Taken together, these findings challenge the direct stimulatory nature of creatine beyond an in vitro model and suggest that CKMT1 is not a primary contributor of EE. en_US
dc.language.iso en en_US
dc.publisher University of Guelph en_US
dc.subject adipose tissue en_US
dc.subject non-shivering thermogenesis en_US
dc.subject futile creatine cycling en_US
dc.subject CKMT1 en_US
dc.title Ablation of CKMT1 Does Not Affect Energy Expenditure, Adipose Tissue Mitochondrial Respiration or Susceptibility to High-fat Diet-induced Obesity en_US
dc.type Thesis en_US
dc.degree.programme Human Health and Nutritional Sciences en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Human Health and Nutritional Sciences en_US
dc.description.embargo 2022-07-22
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.degree.grantor University of Guelph en_US


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