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Weeding Out Challenges in Cannabis Tissue Culture and Developing Novel Micropropagation and Regeneration Methods

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Title: Weeding Out Challenges in Cannabis Tissue Culture and Developing Novel Micropropagation and Regeneration Methods
Author: Monthony, Adrian S.
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: Jones, Max
Abstract: Cannabis sativa L. remains under-studied due to years of prohibition and recent research has highlighted the limitations of Cannabis tissue culture methods. Micropropagation and regeneration methods that can reliably be integrated into plant biotechnology pipelines are needed. This thesis explores three aspects of Cannabis tissue culture, which currently lack reliable and replicable methods: the development of high-multiplication rate micropropagation methods, the use of existing leaf-to-plant regeneration method, and the exploration of protoplast-to-plant regeneration systems in Cannabis. The results presented herein, demonstrate that: floral tissues represent a viable alternative to vegetative tissues for the micropropagation of Cannabis; existing methods for regeneration lack the replicability to be used in across a range of representative drug-type genotypes of Cannabis; and that protoplast isolation and cell division from Cannabis callus can be achieved. Taken together, this thesis provides a blueprint for how to design more reliable and replicable Cannabis micropropagation and regeneration methods.
Date: 2021-07
Rights: Attribution 4.0 International
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
Related Publications: Monthony AS, Bagheri S, Zheng Y, Jones AMP (2021) Flower power: floral reversion as a viable alternative to nodal micropropagation in Cannabis sativa. In Vitro Cell Dev Biol - Plant 21:1–13. AS, Page SR, Hesami M, Jones AMP (2021) The Past, Present and Future of Cannabis sativa Tissue Culture. Plants 10:185.

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Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International